Assessment of dynamic thiol-disulfide homeostasis in patients with lipoid proteinosis (Urbach-Wiethe syndrome)

SUMMARY OBJECTIVE: Lipoid proteinosis is a rare autosomal recessive genetic dermatological disease that occurs due to the accumulation of hyaline material in the skin and mucous membranes. This study aimed to investigate whether dynamic thiol-disulfide homeostasis is a new marker of oxidative stress in patients suffering from lipoid proteinosis. METHODS: The study group involved 17 patients with lipoid proteinosis and 17 healthy controls with same gender and age. Native thiol, total thiol, disulfide levels, and thiol-disulfide indexes were measured with the fully automated spectrophotometric method described by Erel and Neselioglu, and the results of the two groups were statistically analyzed. RESULTS: Serum total thiol and native thiol levels were significantly lower in lipoid proteinosis group compared to the control group (p=0.020 and p=0.014, respectively). The disulfide levels were found to be higher in lipoid proteinosis group, but there was no significant difference between two groups. CONCLUSIONS: Impaired dynamic thiol-disulfide homeostasis was observed in lipoid proteinosis patients, suggesting that thiol-disulfide homeostasis may have a role in the pathogenesis of this disease.

Comparison of the effects of intrathecal different dosage of levobupivacaine in elective day-case arthroscopy of the knee

To observe the motor and sensorial block characteristics with different dosage of levobupivacaine during spinal block for the patients undergoing day-case knee arthroscopy. Eighty, 80 ASA physical status I-II patients, undergoing day-case knee arthroscopy received 0.5% levobupivacaine; 7.5 mg in Group I, 10 mg in Group II, 12.5 mg in Group III and 15mg in Group IV for spinal anesthesia. Maximum sensorial and motor block levels, sensorial and motor block durations, time to required readiness to surgery after block and side effects were recorded. The time required to achieve readiness to surgery was longest and mean duration of sensory and motor block was shortest in Group I. The groups were similar in regards to the number of failed blocks requiring general anaesthesia [p>0.05]. First micturition and unassisted ambulation with crutches times were shortest in Group I [p<0.01]. Home discharge and first additional analgesic request time were similar in four group [p>0.05]. The motor and sensorial block obtained with different spinal anesthesia dosages of 0.5% levobupivacaine were effective for day-case knee artroscopy. Although 7.5 mg is suitable for patients; 10, 12.5 and 15 mg could be used according to anesthetist experience without any side effects